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KMID : 0381219750070050333
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Journal of RIMSK
1975 Volume.7 No. 5 p.333 ~ p.338
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Electron Microscope Autoradiography
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Abstract
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Liquier-Milward, working at Birmingham University, was probably the first to couple autoradiography with electron microscopy.
Electon microscope autoradiography is rapidly coming into its own as a cytochemical tool. It is . employed to localize and quantify radioactive material at submicroscopic levels. The higher autoradiographic resolution, although still far from that of the electron micros
cope itself, provides a more accurate localization of intracellular radioactivity than is possible with the light microscope technique.
The developed grains in the emulsion are distinctly seen and are in focus at the same time as the biological tissue. Grain counting for quantitative work is thereby made easy.
The cytological details which are resolved in the electron microscope facilitate the id- nti ti cation of tissue structures which can then be correlated with developed grain density b establish the most likely source of radioactivity.
Both electron microscope and light microscope autoradiography are based on the same phy sical principles since both use a thin layer of photographic emulsion closely applied to th specimen as a radiation detector.
However, in EM autoradiography, the larger magnification and higher resolution, the scalinj down of the section and emulsion thicknesses, and the need for extra fine grained emulsion to obtain optimum autoradiographic resolution, accentuate some of the difficulties familiar t, the light microscopic autoradiographer while introducing new ones unique to itself.
The basic technique involves the preparation and mounting of a specimen section on support, coating it with emulsion, storing it for exposure, developing it, and analyzing th resultant autoradiograms.
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